Project 4

Embryo-maternal communication disruption during in vitro production (IVP)

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Host Institution:

CSIC-INIA (Madrid,Spain)

Doctoral Candidate: Gislaine Dos Santos

Supervisor: Dr. Dimitrios Rizos

Co-supervisor: Dr. Patrick Lonergan | Dr. Laura Abril Parreño

Non-academic mentor: Dr. Chrystelle Ledanvic

Institution of enrolment:

Other institutions involved/secondments:

Enrolment in Doctoral degrees: UMU (Biology and Technology of Reproductive Health)-UCD (Reproductive Biology)

OBJECTIVES

Scrutinize the miRNA content of the oviductal and uterine extracellular vesicles (EVs) in vivo in response to the embryo presence at key stages of preimplantation development (Day 3.5: 8-16 cell; Day 7: blastocyst).
Determine the miRNA targets through functional enrichment analysis and the identify of regulatory pathways affected by these miRNAs' altered expression.
Re-establishing embryo-maternal communication in vitro through the supplementation and transfection of key miRNAs during early embryo development.
Evaluate the capacity of in vitro produced embryos leading to successful pregnancy outcomes after transfer to recipients.