Project 5

Epitranscriptomic impact in IVF derived preimplantation embryos

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Host Institution:

Univ. Murcia (Murcia,Spain)

Doctoral Candidate: Alessandro Volsa

Supervisor: Dr. Sebastian Canovas

Co-supervisors: Dr. Pablo Ross | Dr. Eva Hoffman

Institution of enrolment:

Other institutions involved/secondments:

Enrolment in Doctoral degrees: UMU (Biology and Technology of Reproductive Health)- UCPH (Cellular and genetic Medicine)

OBJECTIVES

Analyse N⁶-methyladenosine (m⁶A) methylation in bovine embryos as representative of the epitranscriptome as it is the most common modification of mRNA in mammalian cells.
Decipher if there are significant differences in m⁶A profile between in vivo derived and IVF derived bovine embryos.
Assay if during early embryo development, mainly around embryo genome activation (8cells in cow) exist crosstalk between RNA methylation (m⁶A) and histone/DNA epigenetic mechanisms.
Test how culture media composition affects the m⁶A signature in bovine embryos.